Direct somatic embryogenesis from protoplasts of Citrus mitis Blanco
Identifieur interne : 004033 ( Main/Exploration ); précédent : 004032; suivant : 004034Direct somatic embryogenesis from protoplasts of Citrus mitis Blanco
Auteurs : Guek-Eng Sim [Singapour] ; Chiang-Shiong Loh [Singapour] ; Chong-Jin Goh [Singapour]Source :
- Plant Cell Reports [ 0721-7714 ] ; 1988-10-01.
Abstract
Abstract: Protoplasts isolated from embryogenic suspension cultures of Citrus mitis were cultured in a medium without any plant growth substances. Somatic embryos developed directly from protoplasts without an obvious intervening callus phase. As many as 1,800 somatic embryos developed from 4 ml of protoplast suspension (density 2×106/ml) cultured for 35 days. Upon transferring the embryoids to medium with 1 mgl−1 GA3, they developed into plant-lets. Rooted plantlets were obtained in 3 months after protoplast isolation.
Url:
DOI: 10.1007/BF00269527
Affiliations:
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Le document en format XML
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<front><div type="abstract" xml:lang="en">Abstract: Protoplasts isolated from embryogenic suspension cultures of Citrus mitis were cultured in a medium without any plant growth substances. Somatic embryos developed directly from protoplasts without an obvious intervening callus phase. As many as 1,800 somatic embryos developed from 4 ml of protoplast suspension (density 2×106/ml) cultured for 35 days. Upon transferring the embryoids to medium with 1 mgl−1 GA3, they developed into plant-lets. Rooted plantlets were obtained in 3 months after protoplast isolation.</div>
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